Thermo Fisher Scientific SBP2 Polyclonal Antibody
SBP2 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 폴리클로날 항체. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합. 인간 및 생쥐 반응성. 합성 펩타이드 면역원 기반, 액상 형태로 제공되며 -20°C에서 보관.
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- PA518580
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.3 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (KERQERKQRLQEN) corresponding to the internal amino acids of SECISBP2 (aa 538–550) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10988132 |
Product Specific Information
- Predicted to react with bovine, canine, and porcine based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 64,000.
Target Information
Eukaryotes require a selenocysteine (Sec) insertion sequence (SECIS) element in the 3′ untranslated region of the mRNA to decode the UGA codon as Sec.
SECIS-binding protein 2 (SBP-2) specifically binds selenoprotein mRNAs to form a functional complex and is essential for the insertion of Sec into selenoproteins.
Purified SBP-2 interacts specifically with the SECIS element in the phospholipid hydroperoxide glutathione peroxidase mRNA.
SBP-2 shows binding activity in the liver and testis as well as hepatoma cells.
It binds to a conserved RNA binding domain shared with several ribosomal proteins and eukaryotic translation termination release factor 1.
A second domain located N-terminal to the RNA binding domain, required for Sec insertion, allows SBP-2 to stably associate with the ribosomal fraction of cells.
SBP-2 preferentially stimulates incorporation directed by the selenoprotein P and phospholipid hydroperoxide glutathione peroxidase SECIS elements.
SBP-2 may have a distinct function in selecting the ribosomes for Sec insertion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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