Thermo Fisher Scientific ATF6 Polyclonal Antibody
ATF6 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 실험에 사용 가능. 고순도 항원 친화 크로마토그래피 정제 제품이며, 인간 시료에 반응. 액상 형태로 4°C 보관.
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Thermo Fisher Scientific ATF6 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 620 and 670 of human Activating Transcription Factor 6 (ATF6, UniProt ID: P18850-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
ATF6 is a transcription factor that acts during endoplasmic reticulum (ER) stress by activating unfolded protein response (UPR) target genes.
It binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) and ERSE II sequences. Binding to ERSE requires NF-Y interaction.
ATF6 may also participate in transcription activation via serum response factor. It forms homodimers and heterodimers with ATF6 beta and interacts with NF-Y trimer through NF-YC.
Under ER stress, the cleaved N-terminal cytoplasmic domain translocates into the nucleus to activate transcription.
ATF6 belongs to the bZIP family and is N-glycosylated, phosphorylated by MAPK14/P38MAPK, and processed by site-1 and site-2 proteases.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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