Thermo Fisher Scientific ATF6 Polyclonal Antibody
ATF6 단백질을 표적하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, IP 등 다양한 응용에 적합. 액상 형태로 1 mg/mL 농도, 4°C에서 보관. ER 스트레스 관련 유전자 발현 연구에 활용 가능.
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Thermo Fisher Scientific ATF6 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 620 and 670 of human Activating Transcription Factor 6 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
Target Information
ATF6 is a transcription factor that acts during endoplasmic reticulum (ER) stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) and ERSE II sequences. Binding to ERSE requires NF-Y interaction.
ATF6 exists as a homodimer and heterodimer with ATF6 beta and interacts with NF-Y, GTF2I, YY1, and SRF. Under ER stress, the cleaved N-terminal cytoplasmic domain translocates into the nucleus, functioning as a transcription factor. The basic domain serves as a nuclear localization signal, and the leucine-zipper domain mediates NF-Y binding.
During the unfolded protein response, a ~50 kDa fragment containing the transcription factor domain is released by proteolysis, performed by site-1 and site-2 proteases. ATF6 is N-glycosylated and phosphorylated in vitro by MAPK14/P38MAPK, belonging to the bZIP family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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