Thermo Fisher Scientific ATF6 Polyclonal Antibody

Thermo Fisher Scientific ATF6 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections

Target Information

ATF6 is a transcription factor that acts during endoplasmic reticulum (ER) stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) and ERSE II sequences. Binding to ERSE requires NF-Y interaction.
ATF6 exists as a homodimer and heterodimer with ATF6 beta and interacts with NF-Y, GTF2I, YY1, and SRF. Under ER stress, the cleaved N-terminal cytoplasmic domain translocates into the nucleus, functioning as a transcription factor. The basic domain serves as a nuclear localization signal, and the leucine-zipper domain mediates NF-Y binding.
During the unfolded protein response, a ~50 kDa fragment containing the transcription factor domain is released by proteolysis, performed by site-1 and site-2 proteases. ATF6 is N-glycosylated and phosphorylated in vitro by MAPK14/P38MAPK, belonging to the bZIP family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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