Thermo Fisher Scientific UNG Monoclonal Antibody (OTI2F3), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100

Product Specifications

Target Information

The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These forms are generated by alternative splicing and the use of two differentially regulated promoters, PA and PB.
UNG1 and UNG2 cDNAs are similar in size (2061 and 2058 bp), resulting in unresolved mRNA species in gel electrophoresis.
Nuclear UNG2 differs from mitochondrial UNG1 by 44 amino acids in the N-terminal sequence, which is not required for catalytic activity.
UNG gene products play a major role in repairing mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues.
Expression levels of UNG1 and UNG2 vary by cell and tissue type and are regulated by the cell cycle.
UNG1 is a 304 amino acid protein, while UNG2 is a 313 amino acid protein.
Expression and activity are generally higher in proliferating cells and tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.