Thermo Fisher Scientific UNG Monoclonal Antibody (OTI2C12), TrueMAB
Thermo Fisher Scientific의 UNG Monoclonal Antibody (OTI2C12)는 인간 UNG 단백질을 인식하는 Mouse IgG2b 항체로, WB, IHC(P), ICC/IF에 사용 가능. 고순도 Affinity chromatography 정제, 안정적 PBS/BSA/glycerol 저장 버퍼 구성, 연구용으로 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2C12 |
| Immunogen | Full length human recombinant protein of human UNG produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.42 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These forms are generated by alternative splicing and the use of two differentially regulated promoters, PA and PB.
The cDNAs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively) and are not resolved as two species in gel electrophoresis.
Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-terminal sequence that is not necessary for catalytic activity.
A major role of the UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues.
The level and expression pattern of UNG1 and UNG2 differs between cell and tissue type, and the expression of UNG is cell cycle regulated.
Expression and activity of UNG are generally higher in proliferating compared to nonproliferating tissues and cells.
UNG1 is a 304 amino acid protein, and UNG2 is a 313 amino acid protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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