Thermo Fisher Scientific ATP6V1F Monoclonal Antibody (OTI1B8), TrueMAB
ATP6V1F 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, Western Blot, IHC, ICC/IF, Flow Cytometry에 사용 가능. 고순도 Affinity chromatography 정제, 안정적인 PBS/BSA/glycerol buffer 제공. 연구용으로 세포 내 산성화 관련 단백질 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B8 |
| Immunogen | Full length human recombinant protein of human ATP6V1F produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ATP6V1F encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles.
V-ATPase-dependent organelle acidification is necessary for intracellular processes such as:
- Protein sorting
- Zymogen activation
- Receptor-mediated endocytosis
- Synaptic vesicle proton gradient generation
V-ATPase is composed of:
- V1 domain (cytosolic): three A, three B, two G subunits, plus C, D, E, F, and H subunits (contains ATP catalytic site)
- V0 domain (transmembrane): subunits a, c, c', c'', and d
Additional isoforms of many V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants.
This encoded protein is the V1 domain F subunit protein.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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