Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), Super Bright 436, eBioscience

Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), Super Bright 436, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats Available

• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Red 700
• NovaFluor Yellow 570
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains, recognizing the majority of the TCR V alpha 2 subfamily in mice carrying the a, b, and c haplotypes. It also reacts with TCR V delta 8 products due to high homology.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse lymph node cells at ≤0.5 µg/test (100 µL final volume). Recommended Fc receptor blocking with 0.5 µg purified anti-mouse CD16/32 (Product #14-0161-42). Antibody titration is advised for optimal performance.

Super Bright 436 is excited by the violet laser (405 nm) and emits at 436 nm. Use a 450/50 bandpass filter or equivalent. Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

T-cell receptors (TCR) discriminate foreign from self-peptides presented by MHC class II molecules, crucial for adaptive immunity. TCR recognition of self-peptides is linked to autoimmune diseases and tumor-associated mutations. Superantigen engagement of TCRs can cause toxic shock syndrome. Autoantibodies to TCR V beta segments are found in rheumatoid arthritis and SLE, potentially modulating inflammatory responses. Most human T cells express TCR alpha-beta and either CD4 or CD8; gamma-delta T cells, found mainly in epithelia, exhibit distinct antigen recognition and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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