Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), Super Bright 436, eBioscience
마우스 TCR Vα2 체인을 인식하는 B20.1 단클론 항체로, Super Bright 436 형광체가 결합되어 Flow Cytometry 분석에 적합합니다. Violet laser(405 nm)로 여기되며 436 nm에서 방출합니다. 고순도 친화 크로마토그래피 정제 및 안정적 PBS 기반 저장 용액 제공.
- 카탈로그번호
- 62-5812-8x (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), Super Bright 436, eBioscience
Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), Super Bright 436, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.5 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, lambda |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B20.1 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735081 |
Additional Formats Available
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- FITC
- NovaFluor Blue 610-70S
- NovaFluor Red 700
- NovaFluor Yellow 570
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 780
- Request custom conjugation
Product Specific Information
The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains, recognizing the majority of the TCR V alpha 2 subfamily in mice carrying the a, b, and c haplotypes. It also reacts with TCR V delta 8 products due to high homology.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse lymph node cells at ≤0.5 µg/test (100 µL final volume). Recommended Fc receptor blocking with 0.5 µg purified anti-mouse CD16/32 (Product #14-0161-42). Antibody titration is advised for optimal performance.
Super Bright 436 is excited by the violet laser (405 nm) and emits at 436 nm. Use a 450/50 bandpass filter or equivalent. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
T-cell receptors (TCR) discriminate foreign from self-peptides presented by MHC class II molecules, crucial for adaptive immunity. TCR recognition of self-peptides is linked to autoimmune diseases and tumor-associated mutations. Superantigen engagement of TCRs can cause toxic shock syndrome. Autoantibodies to TCR V beta segments are found in rheumatoid arthritis and SLE, potentially modulating inflammatory responses. Most human T cells express TCR alpha-beta and either CD4 or CD8; gamma-delta T cells, found mainly in epithelia, exhibit distinct antigen recognition and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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