Thermo Fisher Scientific pro MMP9 Recombinant Rabbit Monoclonal Antibody (2N4X3)
Recombinant rabbit monoclonal antibody against mouse pro MMP9. Validated for WB and ELISA. High specificity and purity with Protein A purification. Supplied in PBS with glycerol for stable storage at -20°C. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2N4X3 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20–107 of mouse MMP9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.23 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
Product Specific Information
Immunogen Sequence:
APYQRQPTFV VFPKDLKTSN LTDTQLAEAY LYRYGYTRAA QMMGEKQSLR PALLMLQKQL SLPQTGELDS QTLKAIRTPR CGVPDVGR
Target Information
MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase belonging to a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. It is secreted as a 92 kDa zymogen, and cleavage of pro-MMP9 results in an active enzyme (~82 kDa).
MMP9 features:
- Gelatin-binding domain with three fibronectin type II units
- Catalytic domain containing a zinc-binding site
- Proline-rich collagen-homologous domain
- Hemopexin-like domain
MMP9 is expressed by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, and endothelial cells. It plays key roles in:
- Inflammatory responses
- Tissue remodeling and wound healing
- Tumor growth and metastasis
Elevated MMP9 levels are associated with idiopathic atrial fibrillation and aortic aneurysm. Studies also suggest involvement in IL-8-induced mobilization of hematopoietic progenitor cells and tumor-associated tissue remodeling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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