Thermo Fisher Scientific LDHA Polyclonal Antibody
LDHA 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IHC, ICC/IF, IP에 사용 가능. 인간 반응성, 다양한 종에 예측 반응. 항원 친화 크로마토그래피로 정제, 4°C 또는 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| Immunoprecipitation (IP) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Lactate Dehydrogenase. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10982548 |
Product Specific Information
- Heat-mediated antigen retrieval is recommended prior to tissue staining.
- This antibody is predicted to react with mouse, rat, rabbit, chimpanzee, and Chinese hamster based on sequence homology.
Target Information
Lactate dehydrogenase (LDH) is an enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+.
In mammals, LDH is encoded by three genes: LDHA (M form), LDHB (H form), and LDHC (X form).
Human LDHs share 84–89% sequence similarity and 69–75% amino acid identity.
There are five LDH isoenzymes based on the combination of M and H chains in the tetrameric structure.
LDHA is hypoxia-inducible and regulated by HIF1α activity.
LDHB is upregulated in many cancers.
Mutations in LDHA can cause Glycogen Storage Disease 11 (GSD11).
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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