Thermo Fisher Scientific Nestin Polyclonal Antibody
Human Nestin 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF 등에 사용 가능. 신경줄기세포 및 종양 내 신생혈관 표지에 활용. 항원 친화 크로마토그래피 정제, 0.2 mg/mL 농도의 액상 형태. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,500–1:5,000 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NES. Recombinant protein control fragment (Product #RP-89156). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2790061 |
Product Specific Information
Immunogen sequence:
EPLRSLEDEN KEAFRSLEKE NQEPLKTLEE EDQSIVRPLE TENHKSLRSL EEQDQETLRT LEKETQQRRR SLGEQDQMTL RPPEKVDLEP LKSLDQEIAR PLENENQEFL KSLKEESVEA VKSLETEILE SLKSAGQENL ETLK
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells.
It is a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Nestin is also a superior angiogenic marker used to evaluate neovascularity of endothelial cells in tumors.
Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, enhance the therapeutic potential of human neural stem/progenitor cells in CNS disease or injury, and identify neuroepithelial tumor cells.
Nestin expression has been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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