Thermo Fisher Scientific Nucleolin/NCL Polyclonal Antibody
Nucleolin 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 응용에 적합. Human과 Mouse 반응성 확인됨. 항원 친화 크로마토그래피로 정제된 액상 제품이며, 4°C에서 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Nucleolin/NCL Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 550 and the C-terminus (residue 710) of human Nucleolin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
Nucleolin (NCL) is a eukaryotic nucleolar phosphoprotein involved in ribosome synthesis and maturation. It is mainly located in dense fibrillar regions of the nucleolus. The human NCL gene consists of 14 exons and 13 introns spanning approximately 11 kb. Intron 11 encodes a small nucleolar RNA termed U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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