Thermo Fisher Scientific Nucleolin/NCL Polyclonal Antibody
Rabbit polyclonal antibody targeting human Nucleolin (NCL). Validated for WB, IHC, IHC-P, and IP applications. Recommended for research use only. Provides consistent performance and high specificity in nucleolar protein detection.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 550 and the C-terminus (residue 710) of human Nucleolin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
Nucleolin (NCL) is a eukaryotic nucleolar phosphoprotein involved in the synthesis and maturation of ribosomes. It is primarily located in dense fibrillar regions of the nucleolus. The human NCL gene consists of 14 exons and 13 introns, spanning approximately 11 kb. Intron 11 encodes a small nucleolar RNA, termed U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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