Thermo Fisher Scientific NOD1 Polyclonal Antibody
NOD1 단백질을 인식하는 Rabbit Polyclonal Antibody로 인간 시료에 반응. Western blot 및 Immunocytochemistry에 사용 가능. 고순도 항원 친화 크로마토그래피 정제. 1 mg/mL 농도, 액상 형태로 제공되며 4°C 또는 -20°C에서 안정적으로 보관 가능.
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Thermo Fisher Scientific NOD1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 698–953 of NOD1 (Uniprot ID: Q9Y239) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11152977 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Raji
- Predicted reactivity: Mouse (81%), Rat (82%), Pig (87%), Bovine (87%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
NOD1 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins involved in innate immune defense. It contains:
- N-terminal caspase recruitment domain (CARD)
- Centrally located nucleotide-binding domain (NBD)
- Ten tandem leucine-rich repeats (LRRs) in its C-terminus
The CARD domain participates in apoptotic signaling, activating caspase-9 and NF-kappa-B. LRRs mediate protein-protein interactions. Mutations in the NBD may impact oligomerization and LRR function. NOD1 acts as an intracellular pattern-recognition receptor (PRR) detecting bacterial diaminopimelic acid to initiate inflammation.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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