Thermo Fisher Scientific IL-6 Mouse ProcartaPlex Simplex Kit

Thermo Fisher Scientific IL-6 Mouse ProcartaPlex™ Simplex Kit

The Mouse IL-6 Simplex ProcartaPlex™ Kit measures IL-6 protein and is designed to be combinable with other Simplex kits, enabling creation of customized multiplex panels utilizing Luminex xMAP technology for protein detection and quantitation.

When combining multiple Simplex kits (i.e., not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is required per assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument.

ProcartaPlex multiplex assays require as little as:

• 25 μL of plasma or serum, or
• 50 μL of cell culture supernatant

Results can be obtained in just four hours.

Key Features

• More results per sample — measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology — highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by Luminex detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX).

Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE).

The main difference from a traditional ELISA is that the capture antibody is conjugated to a bead rather than adsorbed to the microplate well, allowing reagents to remain free-floating in solution.

For detection, the Luminex 200 instrument uses two lasers:

1. To distinguish the spectral signature of each bead
2. To quantify RPE fluorescence, proportional to the amount of protein present in the sample

ProcartaPlex multiplex assays can profile more target proteins using significantly less sample and time compared to traditional sandwich ELISA methods.

ProcartaPlex multiplex panels are available across six species: human, mouse, rat, nonhuman primate, porcine, and canine.
For additional information, visit thermofisher.com/procartaplex.

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