Thermo Fisher Scientific Blood Group H Antigen Monoclonal Antibody (89-8)

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

-

Agglutination (AGG)

1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

89-8

Immunogen

H human blood protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.01% sodium azide

Storage conditions

4°C short term, -80°C long term

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1070775

Product Specific Information

MA1-7674 detects H1 and H2 human blood from human samples.

MA1-7674 has been successfully used in ELISA and agglutination applications.

The MA1-7674 immunogen is a H human blood protein.

For short term storage, store product at 4ºC. For long-term storage, store at -80ºC

Target Information

The H antigen is a precursor to the ABO blood group antigens, present in people of all common blood types. The Bombay phenotype (hh) does not express antigen H on red blood cells, and therefore this type will also lack A or B antigens, similar to the O blood group. However, unlike O group, the H antigen is absent, hence the individuals produce isoantibodies to antigen H as well as to both A and B antigens. If they receive blood from someone with O blood group, the anti-H antibodies will bind to the H antigen on the red blood cells of the donor blood and destroy the RBCs by complement-mediated lysis. Therefore, people with Bombay phenotype can receive blood only from other hh donors, although they can donate as though they were type O. Some individuals with the blood group A1 may also be able to produce anti-H antibodies due to the complete conversion of all the H antigen to A1 antigen. Production of the H antigen, or its deficiency in the Bombay phenotype, is controlled at the H locus on chromosome 19. The H locus contains three exons that span more than 5 kb of genomic DNA, and encodes the fucosyltransferase that produces the H antigen on RBCs. The H antigen is a carbohydrate sequence with carbohydrates linked mainly to protein (with a minor fraction attached to ceramide moiety).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.