Thermo Fisher Scientific RET Monoclonal Antibody (OTI2B3), TrueMAB
RET 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 면역세포염색에 적합합니다. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. 인간 RET 단백질(aa 713–1017) 기반 면역원으로 제작되었습니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 713–1017 of human RET produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
- For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The receptor tyrosine kinase RET is a member of the cadherin superfamily and plays a crucial role in neural crest development. RET can undergo oncogenic activation by cytogenetic rearrangement and transduces signals for cell growth and differentiation.
Mutations in the RET gene are associated with multiple endocrine neoplasia types IIA and IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been identified for the RET gene, with additional variants described but not biologically confirmed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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