Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647

Applications

Tested Dilution

Western Blot (WB)

1:2000-1:10,000

Immunohistochemistry (Paraffin) (IHC (P))

1:500

Immunocytochemistry (ICC/IF)

0.5-10 µg/mL

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

View additional formats

• [Agarose](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Agarose/host/Goat/)
• [Alexa Fluor 350 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 350/host/Goat/)
• [Alexa Fluor 405 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 405/host/Goat/)
• [Alexa Fluor 430 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 430/host/Goat/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 514 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 514/host/Goat/)
• [Alexa Fluor 532 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 532/host/Goat/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 546/host/Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Goat/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Goat/)
• [Alexa Fluor 633 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 633/host/Goat/)
• [Alexa Fluor 635 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 635/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor 660 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 660/host/Goat/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 680/host/Goat/)
• [Alexa Fluor 700 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 700/host/Goat/)
• [Alexa Fluor 750 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 750/host/Goat/)
• [Alexa Fluor 790 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 790/host/Goat/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 555 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 555/host/Goat/)
• [Alexa Fluor Plus 594 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 594/host/Goat/)
• [AP](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/AP/host/Goat/)
• [APC ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/APC/host/Goat/)
• [APC-Alexa Fluor 750](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/APC-Alexa Fluor 750/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Biotin/host/Goat/)
• [Biotin-XX](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Biotin-XX/host/Goat/)
• [BODIPY FL](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/BODIPY FL/host/Goat/)
• [Cyanine3](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Cyanine3/host/Goat/)
• [Cyanine5 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Cyanine5/host/Goat/)
• [DyLight 350](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 350/host/Goat/)
• [DyLight 405](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 405/host/Goat/)
• [DyLight 488](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 488/host/Goat/)
• [DyLight 550](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 550/host/Goat/)
• [DyLight 594](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 594/host/Goat/)
• [DyLight 633](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 633/host/Goat/)
• [DyLight 650](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 650/host/Goat/)
• [DyLight 680](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 680/host/Goat/)
• [DyLight 755](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 755/host/Goat/)
• [DyLight 800](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 800/host/Goat/)
• [FITC](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Goat/)
• [Pacific Blue](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Pacific Blue/host/Goat/)
• [Pacific Orange](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Pacific Orange/host/Goat/)
• [PE ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/PE/host/Goat/)
• [PE-Alexa Fluor 610](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/PE-Alexa Fluor 610/host/Goat/)
• [Rhodamine](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Rhodamine/host/Goat/)
• [Rhodamine Red-X](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Rhodamine Red-X/host/Goat/)
• [Texas Red-X](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Texas Red-X/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/TRITC/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

658/675 nm View spectra

Form

Liquid

Concentration

2 mg/mL

Purification

Affinity chromatography

Storage buffer

proprietary buffer, pH 6.5

Contains

0.016% Methylisothiazolone, 0.016% Bromonitrodioxane

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2896355

Target

IgG

Cross Adsorption

human, horse, bovine

Antibody Form

F(ab`)2 Fragment

Product Specific Information

F(ab`)2 antibody fragments offer several benefits over whole IgGs, due to their smaller size (~110 kD vs. ~ 150 kD) and the absence of most of the Fc region. The smaller size allows for better penetration into tissue sections, resulting in improved staining in applications such as IHC. The lack of Fc region results in reduced nonspecific background staining when staining cells with Fc receptors (such as immune cells) and other Fc interaction-related interference.

F(ab`)2 antibody fragments were obtained by a combination of pepsin digestion and immunoaffinity chromatography to remove residual Fc fragments and undigested whole IgG molecules.

To minimize cross-reactivity, the goat anti-mouse IgG F(ab`)2 fragments have been cross-adsorbed against human, horse and bovine serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.

Specificity: This antibody binds to heavy chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody does not bind to human, horse or bovine serum proteins/IgG or mouse non-immunoglobulin serum proteins.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.