Ion Torrent™

Oncomine™ Comprehensive Assay Plus, manual library preparation

The Oncomine Comprehensive Assay Plus is a targeted, next-generation sequencing (NGS) assay that provides a comprehensive genomic profiling solution appropriate자세히 알아보기

The Oncomine Comprehensive Assay Plus is a targeted, next-generation sequencing (NGS) assay that provides a comprehensive genomic profiling solution appropriate for formalin-fixed paraffin-embedded (FFPE) tissues from solid tumors. The assay detects multiple biomarkers associated with targeted and immune checkpoint therapies for cancer research. It allows concurrent analysis of DNA and RNA to simultaneously detect multiple types of variants in a single workflow, including single nucleotide variants (SNVs), insertions and deletions (InDels), copy number variants (CNVs), BRCA1 and BRCA2 large genomic rearrangements (LGRs), genomic instability metric (GIM), microsatellite instability (MSI), and tumor mutational burden (TMB), loss-of-heterozygosity (LOH), and gene fusions featuring FusionSync.

Key features of the assay include:

• Profile 500+ unique genes for single gene and multi-gene biomarker insights

• Enables analysis of various single-gene variants, such as SNVs, indels, fusions, splice variants, and CNV, including both copy number gains and losses

• Detection of complex biomarkers associated with immunotherapies such as TMB and MSI

• Supports homologous recombination deficiency (HRD) research by detecting mutations in 46 HRR genes, including BRCA LGRs, and assessment genomic scarring with the genomic instability metric (GIM)

• Robust performance from as little as 20 ng of DNA/RNA isolated from FFPE samples including fine needle biopsies

• Content driven by the Oncomine Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published evidence

• Highly automated workflow and streamlined bioinformatics analysis pipeline optimized for Oncomine Comprehensive Assay Plus helps deliver insights

The Oncomine Comprehensive Assay Plus provides the reagents for library construction and four pools—two DNA and two RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Designed to help you go from hundreds down to a few relevant cancer drivers, this assay is part of a comprehensive workflow that enables NGS data analysis from as little as 10 ng of nucleic acid per pool. The assay is optimized for sequencing on the Ion GeneStudio S5 systems with the Ion 550 Chip to enable up to four samples and a no-template control (NTC) per run.

When combined with Oncomine Reporter Software, you can focus on key drivers of cancer in your research.

From sample to answer
The Oncomine Comprehensive Assay Plus is part of an NGS workflow that helps researchers filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine Informatics, this filtering helps you contextualize and interpret findings to move toward the answers you need.

Learn more about Oncomine Oncology ›

사양

샘플 종류DNA, RNA

Sequencing TypeNext Generation Sequencing

용도(장비)Ion GeneStudio™ S5 Prime System, Ion GeneStudio™ S5 Plus System, Ion S5™ XL System, Ion Chef™ Instrument

라이브러리Targeted Sequencing Library (Amplicon based)

제품라인Oncomine

수량24 Samples

워크플로우 단계Library Generation

Unit SizeEach