Thermo Fisher Scientific PCK1 Polyclonal Antibody
Thermo Fisher Scientific의 PCK1 폴리클로날 항체는 인간에 반응하며, Western blot 및 IHC(Paraffin) 실험에 사용됩니다. 합성 펩타이드 항원 기반으로 제작되었으며, 액상 형태로 0.5 mg/mL 농도입니다. 연구용으로만 사용 가능하며 다양한 종에 교차 반응이 예측됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 0.3 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (HVNWFRKDKEGK) corresponding to internal amino acids of PCK1 (aa 513–525) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10981753 |
Product Specific Information
This antibody is predicted to react with bovine, canine, mouse, porcine, and rat based on sequence homology.
Tested in Peptide ELISA: antibody detection limit dilution 8,000.
Target Information
Phosphoenolpyruvate Carboxykinase 1 (PCK1) is a main control point for the regulation of gluconeogenesis. During gluconeogenesis, PCK1 acts as the rate-limiting enzyme. It regulates the formation and maintenance of memory CH8(+) T-cells via gluconeogenesis and controls levels of metabolic intermediates in the citric acid cycle by regulating cataplerosis and anaplerosis.
When glucose levels are high, PCK1 catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP); when low, it catalyzes the anaplerotic conversion of PEP to oxaloacetate. Phosphorylation at Ser-90 by AKT1 enables PCK1 to act as a protein kinase, promoting lipogenesis via SREBP activation.
An important paralog of this gene is PCK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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