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Takara Exonuclease I
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Takara Exonuclease I

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단일가닥 DNA 분해에 사용되는 효소로, PCR 반응 후 프라이머 제거에 적합합니다. DNA end blunting에는 부적절하며, 별도의 효소를 사용해야 합니다. 10X Exonuclease I Buffer(670 mM Glycine-KOH 등) 포함. 연구용 전용 제품입니다.

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Enzymes
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pk
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Takara 2650A Exonuclease I, 750 Units pk판매 단위 pk ·
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Takara 2650B Exonuclease I, 3,750 Units pk판매 단위 pk ·
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Takara · Takara Exonuclease I

Takara Exonuclease I

Applications

  • Single-stranded DNA degradation
  • Removal of ssDNA fragments in a reaction mixture
  • PCR primer digestion post-reaction

Notes

Exonuclease I should not be used for DNA end blunting; short DNA ends are not an appropriate substrate for Exonuclease I.
For DNA end blunting, use DNA Polymerase I, Klenow Fragment (2140A, 2140AK, 2140B, 2140BK) or Mung Bean Nuclease (2420A, 2420B) instead.

Buffer Composition

Component Concentration pH Additional Info
Glycine-KOH 670 mM 9.5
DTT 10 mM
MgCl₂ 67 mM

Supplied with 1 ml 10X Exonuclease I Buffer.

Additional Product Information

Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications.
Refer to the Kit Components List for details on included materials.
Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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