Applications
- Single-stranded DNA degradation
- Removal of ssDNA fragments in a reaction mixture
- PCR primer digestion post-reaction
Notes
Exonuclease I should not be used for DNA end blunting; short DNA ends are not an appropriate substrate for Exonuclease I. For DNA end blunting, use DNA Polymerase I, Klenow Fragment (2140A, 2140AK, 2140B, 2140BK) or Mung Bean Nuclease (2420A, 2420B) instead.
Buffer
Supplied with 1 ml 10X Exonuclease I Buffer [670 mM Glycine-KOH, pH 9.5, 10 mM DTT, 67 mM MgCl2].
Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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