Takara T4 RNA Ligase

T4 RNA Ligase is a ligation enzyme for joining single-stranded RNA or DNA fragments by catalyzing the formation of phosphodiester bonds between single-stranded RNA or DNA containing 5-phosphate and 3-hydroxyl termini. This enzyme requires ATP for activity, and its activity is highest for RNA-RNA ligation, lower for RNA-DNA ligation, and lowest for DNA-DNA ligation. 3-end labeling is often completed using 5-[32P]pCp.

More Information

Applications

• Ligation of single-stranded RNA or DNA
• 3`-end labeling of single stranded RNA or DNA

Source

E. coli B strain infected with T4 am N82

Storage

–20°C

Concentration

10–50 U/µl

Unit definition

One unit is defined as the amount of enzyme that converts 1 pmol of [5-32P\]pCp into its acid-insoluble form in 10 minutes at 5°C, using oligo(A) as the substrate during 3 end labeling of RNA.

Product citations

England, T. E., Bruce, A. G. & Uhlenbeck, O. C. Specific labeling of 3` termini of RNA with T4 RNA ligase. Methods Enzymol. 65, 65–74 (1980).

Romaniuk, P. J. & Uhlenbeck, O. C. Joining of RNA molecules with RNA ligase. Methods Enzymol. 100, 52–9 (1983).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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