Thermo Fisher Scientific PFTK2 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended positive controls: H1299, HepG2
• Predicted reactivity: Mouse (94%)
• Store product as a concentrated solution.
• Centrifuge briefly prior to opening the vial.

Target Information

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the γ phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating processes such as metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, cell movement, apoptosis, and differentiation.

With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. They are classified into eight major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the MAPK cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs), have been best characterized in the yeast pheromone response pathway.

Safety Information

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.