Thermo Fisher Scientific Phospho-DARPP-32 (Thr34) Polyclonal Antibody
Phospho-DARPP-32 (Thr34) 특이적 폴리클로날 항체로, Western blot, IHC, ICC 등 다양한 응용에 적합합니다. Rabbit IgG 기반으로 1 mg/mL 농도의 액상 형태이며, -20°C에서 보관합니다. 도파민 신호 조절 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | – |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphopeptide derived from human DARPP-32 around the phosphorylation site of Thr34 (R-P-TP-P-A) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2556244 |
Target Information
DARPP-32 is a dopamine (DA) and AMP-regulated ~32 kDa phosphoprotein associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when phosphorylated on Thr34. Conversely, phosphorylation on Thr75 converts DARPP-32 into a PKA inhibitor. Phosphorylation of DARPP-32 plays a critical role in dopaminergic neurotransmission and is implicated in the actions of alcohol, caffeine, and Prozac®.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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