Thermo Fisher Scientific Phospho-ELK1 (Ser389) Polyclonal Antibody
Rabbit polyclonal antibody recognizing phosphorylated ELK1 (Ser389). Validated for WB, IHC-P, and IP applications. Supplied unconjugated in liquid form at 1 mg/mL. Suitable for research use in MAPK signaling and transcriptional regulation studies.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphopeptide derived from human Elk1 around the phosphorylation site of Ser389 (P-R-SP-P-A) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2556245 |
Target Information
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. It is phosphorylated by MAP kinase pathways at a cluster of serine/threonine motifs at its C-terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by ELK1. ELK1 acts as a direct target of activated MAP kinase and serves as a nuclear effector in the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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