Thermo Fisher Scientific TRAF5 Polyclonal Antibody
Human TRAF5 단백질에 특이적인 Rabbit Polyclonal 항체. IHC(P) 및 ICC/IF에 사용 가능하며, 항원 친화 크로마토그래피로 정제됨. PBS/glycerol buffer에 보존되어 장기 안정성 우수. 연구용으로 TNF 수용체 신호전달 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human TRAF5. Recombinant protein control fragment (Product # RP-89647) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2648874 |
Product Specific Information
Immunogen sequence:
NAKVILGRYQ QDHLQQCLFQ PVQCSNEKCR EPVLRKDLKE HLSASCQFRK EKCLYCKKDV VVINLQNHEE NLCPEYPVFC PNNCAKIILK TEVDEHLAVC PEAEQDCPFK HYGCAVTDKR
Sequence identity:
- Mouse: 70%
- Rat: 69%
Target Information
Tumor necrosis factor (TNF)-induced signaling is mediated through the association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor-associated factors (TRAFs).
TRAFs are cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g., RANK, CD30, CD40) and the interleukin-1 receptor.
The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization.
Six TRAFs (TRAF1–TRAF6) have been identified. TRAF5 is a 558-amino acid protein implicated in NF-κB and c-Jun N-terminal kinase/stress-activated protein kinase activation by TNF receptor superfamily members, including CD27, CD30, CD40, and lymphotoxin-beta receptor.
Targeted disruption of the TRAF5 gene causes defects in CD40–CD27 mediated lymphocyte activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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