Thermo Fisher Scientific RNF43 Polyclonal Antibody
Human RNF43 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(P) 실험에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관됩니다. Wnt 신호 조절 연구에 활용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human RNF43. Recombinant protein control fragment (Product #RP-89654). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2646680 |
Product Specific Information
Immunogen sequence:
CVDPWLHQHR TCPLCMFNIT EGDSFSQSLG PSRSYQEPGR RLHLIRQHPG HAHYHLPAAY LLGPSRSAVA RPPRPGPFLP SQEPGMGPRH HRFPRAAHPR APGEQQRLAG AQHPYAQGWG LSHLQSTSQH
- Highest antigen sequence identity to the following orthologs:
- Mouse: 78%
- Rat: 79%
Target Information
E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis, and subsequent degradation of Wnt receptor complex components Frizzled.
Acts on both canonical and non-canonical Wnt signaling pathways.
Functions as a tumor suppressor in the intestinal stem cell zone by inhibiting Wnt signaling, thereby restricting the size of the intestinal stem cell zone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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