Thermo Fisher Scientific eIF3J/EIF3S1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunohistochemistry (IHC)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

-

Immunoprecipitation (IP)

2-5 µg/mg lysate

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 208 and 258 of human eukaryotic translation initiation factor 3, subunit J (eukaryotic translation initiation factor 3, subunit 1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:eIF3j,uniProtId:O75822-1,ncbiNodeId:9606,antigenRange:208-258,antigenLength:258,antigenImageFileName:A301-746A-M_eIF3j_O75822-1_Rabbit.svg,antigenImageFileNamePDP:A301-746A-M_eIF3j_O75822-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Rat and Orangutan

Target Information

Eukaryotic initiation factor-3 (EIF3) has a molecular mass of about 600 kD and contains 13 nonidentical protein subunits, including EIF3J. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex (Fraser et al., 2004; Fraser et al., 2007).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.