Thermo Fisher Scientific SET7 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunohistochemistry (IHC)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

-

Immunoprecipitation (IP)

2-5 µg/mg lysate

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 316 and 366 of Human SET domain containing (lysine methyltransferase) 7. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SETD7,uniProtId:Q8WTS6-1,ncbiNodeId:9606,antigenRange:316-366,antigenLength:366,antigenImageFileName:A301-747A-M_SETD7_Q8WTS6-1_Rabbit.svg,antigenImageFileNamePDP:A301-747A-M_SETD7_Q8WTS6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

Histone methyltransferase that specifically monomethylates Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates Lys-189 of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates Lys-372 of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.