Thermo Fisher Scientific SET7 Polyclonal Antibody
SET7 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, IHC, IP 등에 사용 가능. 인간 SETD7(316–366aa) 영역을 면역원으로 제작. 액상 형태로 제공되며 4°C 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 316 and 366 of Human SET domain containing (lysine methyltransferase) 7 (SETD7, UniProt ID: Q8WTS6-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Histone methyltransferase that specifically monomethylates Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activation of transcription.
Also exhibits methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K motif in substrate proteins. Monomethylates Lys-189 of TAF10, increasing its affinity for RNA polymerase II, and monomethylates Lys-372 of p53/TP53, stabilizing p53/TP53 and enhancing its transcriptional activity. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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