Thermo Fisher Scientific RFWD3 Polyclonal Antibody
Human RFWD3 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB 및 IP에 사용 가능. 항원 친화 크로마토그래피로 정제되어 1 mg/mL 농도의 액상 형태로 제공. DNA 손상 복구 관련 연구용으로 적합.
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Applications
Western Blot (WB)
- Assay-dependent
Immunoprecipitation (IP)
- 2–5 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 175 and 225 of Human ring finger and WD repeat domain 3 (RFWD3, UniProt ID: Q6PCD5-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
Target Information
E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signaling and repair by mediating ubiquitination of RPA complex (RPA1, RPA2, RPA3) and RAD51 at stalled replication forks, promoting homologous recombination. Also mediates ubiquitination of p53/TP53, acting as a positive regulator of its stability and the G1/S DNA damage checkpoint. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly restricting MDM2-mediated polyubiquitin chain extension. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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