Thermo Fisher Scientific DDX1 Polyclonal Antibody
DDX1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 항체. 인간, 생쥐, 랫트 반응성. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human DDX1. Recombinant protein control fragment (Product #RP-95989). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2790382 |
Product Specific Information
Immunogen sequence:
NQALFPACVL KNAELKFNFG EEEFKFPPKD GFVALSKAPD GYIVKSQHSG NAQVTQTKFL PNAPKALIVE PSRELAEQTL NNIKQFKKY
Target Information
DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase capable of unwinding both RNA-RNA and RNA-DNA duplexes. It possesses 5′ single-stranded RNA overhang nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides.
DDX1, together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation and positively regulates cyclin CCND2 expression by binding to its promoter region.
It is a component of a multi-helicase-TICAM1 complex that serves as a cytoplasmic sensor of viral double-stranded RNA, playing a role in activation of antiviral responses including induction of proinflammatory cytokines via TICAM1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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