Thermo Fisher Scientific ZEB1 Recombinant Rabbit Monoclonal Antibody (BLR217K)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Flow Cytometry (Flow)

0.25 µL/10^6 cells

-

Immunoprecipitation (IP)

6 µL/mg of lysate

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293F

Class

Recombinant Monoclonal

Type

Antibody

Clone

BLR217K

Immunogen

Peptide representing a region between residue 750 and 800 of Human zinc finger E-box-binding homeobox 1 (ZEB1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ZEB1,uniProtId:P37275-1,ncbiNodeId:9606,antigenRange:750-800,antigenLength:1124,antigenImageFileName:A700-217_ZEB1_P37275-1_Rabbit.svg,antigenImageFileNamePDP:A700-217_ZEB1_P37275-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

BBS, pH 8.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

Target Information

ZEB1 (Zinc finger E-box-binding homeobox 1) acts as a transcriptional repressor. It inhibits interleukin-2 (IL-2) gene expression. ZEB1 enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and depending on the cell type. It represses E-cadherin promoters and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 also represses BCL6 transcription in the presence of the corepressor CTBP1. It postively regulates neuronal differentiation and represses RCOR1 transcription activation during neurogenesis. Mutations in the gene can result in corneal dystrophy, posterior polymorphous and corneal dystrophy, Fuchs endothelial 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.