Thermo Fisher Scientific Neurofilament-L Recombinant Rabbit Monoclonal Antibody (BLR224K)
Neurofilament-L 단백질을 인식하는 재조합 토끼 단클론 항체로, WB, IHC, ICC/IF, IP 등 다양한 응용에 적합합니다. 인간, 생쥐, 랫트 반응성이 있으며, HEK293F 시스템에서 발현된 고순도 항체입니다. 4°C 보관, 연구용 전용 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Immunoprecipitation (IP) | 20 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR224K |
| Immunogen | Peptide representing a region between residue 150 and 200 of Human Neurofilament light |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 250 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunocytochemistry: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE cell sections.
Target Information
Neurofilaments are intermediate filament proteins found specifically in neurons and are composed mainly of three major proteins: NF-L, NF-M, and NF-H.
NF-L (light chain) is the low molecular weight subunit (~70 kDa) that contributes to the maintenance of neuronal caliber.
NF-M and NF-H are medium and heavy molecular weight subunits, respectively, with NF-H typically running on SDS-PAGE gels at 180–220 kDa.
Expression of these proteins is tissue-specific and developmentally regulated.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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