Thermo Fisher Scientific p38 MAPK Polyclonal Antibody
Rabbit polyclonal antibody targeting human p38 MAPK for WB and IP applications. Recognizes residues 300–360 of MAPK14. Supplied as 0.2 mg/mL liquid, stored at 4°C. Predicted cross-reactivity with rat and chimpanzee. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 300 and the C-terminus (residue 360) of human Mitogen Activated Protein Kinase 14 (p38 MAPK) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Rat and Chimpanzee
Target Information
The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other external signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, differing in tissue expression and affinity for upstream activators and downstream effectors. Upon exposure to stimuli such as tumor necrosis factor, interleukin-1, or heat shock, MAPK kinase-3/6 phosphorylate Thr180 and Tyr182 residues in p38 MAPK. Activated p38 MAPK then phosphorylates transcription factors including ATF-2, CHOP-1, and MEF-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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