Thermo Fisher Scientific 4E-T/eIF4E-T Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunohistochemistry (IHC)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

-

Immunoprecipitation (IP)

2-10 µg/mg lysate

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 925 and the C-terminus (residue 985) of human Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:eIF4ENIF1,uniProtId:Q9NRA8-1,ncbiNodeId:9606,antigenRange:925-985,antigenLength:985,antigenImageFileName:A300-706A_eIF4ENIF1_Q9NRA8-1_Rabbit.svg,antigenImageFileNamePDP:A300-706A_eIF4ENIF1_Q9NRA8-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.