Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody

Applications

Tested Dilution

Western Blot (WB)

0.05-1 µg/mL

Immunocytochemistry (ICC/IF)

0.1-2 µg/mL

ELISA (ELISA)

0.01-0.5 µg/mL

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Secondary Antibody

Immunogen

Recombinant full-length protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2536160

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

The sensitivity and specificity of each lot is confirmed using ELISA.

Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig and donkey IgG is observed.

Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.