Thermo Fisher Scientific Phospho-RAD51 (Thr309) Polyclonal Antibody
RAD51 단백질의 Thr309 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human RAD51 around the phosphorylation site of Thr309 [GE(p-T)RI] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recombination. It binds to single- and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments that are essential for the homology search and strand exchange. Mutations in the gene can result in breast cancer, mirror movements 2, and Fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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