Thermo Fisher Scientific ZNF281 Polyclonal Antibody
ZNF281 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, Western blot 및 면역세포화학(ICC/IF)에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS(40% 글리세롤, 0.02% 아지드) 용액 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ZNF281. Recombinant protein control fragment (Product # RP-101420) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2650044 |
Product Specific Information
Immunogen sequence:
SSPLHNHTLF PEKQIYTTSP LECGFGQSVT SVLPSSLPKP PFGMLFGSQP GLYLSALDAT HQQLTPSQEL DDLIDSQKNL ETSSAFQSSS QKLTSQKEQ
Highest antigen sequence identity to the following orthologs:
- Mouse: 100%
- Rat: 100%
Target Information
ZFP281 (also known as ZBP-99) is a member of a conserved family of transcription factors that interact with GC-rich promoters. It contains four Kruppel-like zinc fingers and is highly homologous to ZBP-89, another regulator of ornithine decarboxylase gene expression. ZFP281 interacts with other transcription factors such as Sp1 to repress vimentin gene transcription. It also regulates embryonic stem cell pluripotency by directly controlling specific target genes and interacting with POU5F1, Sox2, and NANOG. Gene expression microarray analysis indicates that ZFP281 can both activate and repress target genes, suggesting bifunctional roles in gene regulation in stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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