Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), NovaFluor Yellow 690, eBioscience

Applications

• Flow Cytometry (Flow): Tested dilution 0.2 µg/test

Product Specifications

Additional Formats Available

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, eFluor series, FITC, PE, PE-Cyanine5/7, Super Bright series, NovaFluor Blue/Red/Yellow variants, and more. Custom conjugation available.

Product Specific Information

The GK1.5 monoclonal antibody recognizes the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, a subset of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays an essential role in T cell development and function. Binding of GK1.5 is blocked by RM4-5.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.2 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize antibody titration for best results.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample or per 100 µL of cell suspension for single-color controls.
  • Add to antibody cocktails before combining with cells.

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered nucleic acid dye structures for optimized fluorescence signatures minimizing spillover and spread.

Excitation/Emission: 552 nm / 690 nm
Laser: 561 nm (Yellow)

Note: Internal testing shows NovaFluor Yellow 690 may non-specifically stain B cells in SJL mice. No such staining observed in BALB/c or C57BL/6 strains.

Target Information

CD4 is a co-receptor for MHC class II recognition and HIV entry. It is expressed in T helper lymphocytes and other immune cells such as monocytes, macrophages, and dendritic cells. CD4 expression is found in thymus, lymph nodes, tonsils, spleen, and some non-lymphoid tissues. It plays a key role in T-cell activation via association with the T-cell receptor complex and tyrosine kinase Lck. Multiple alternatively spliced transcripts exist (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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