Thermo Fisher Scientific Osteopontin Polyclonal Antibody
Osteopontin 단백질을 인식하는 염소 유래 polyclonal 항체로, Western blot 및 IHC(P) 분석에 적합합니다. 인간에 반응하며, 개·마우스·랫에서도 반응이 예측됩니다. 0.5 mg/mL 농도의 액상 형태로 제공되며, -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.3 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the C-terminus amino acids DPKSKEEDKHLK (aa 288–300) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2194968 |
Product Specific Information
- Predicted to react with canine, mouse, and rat based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It plays multifunctional roles in bone mineralization, cell adhesion, migration, chronic inflammatory disease, and cellular transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases near the integrin-binding RGD sequence modulates its binding and function. Thrombin-cleaved fragments are overexpressed in malignant glial tumors, providing survival advantages and acting as substrates for plasmin and cathepsin D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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