Thermo Fisher Scientific Beta-Adducin Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Orangutan

Target Information

Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta, and gamma. These subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of the spectrin–actin network in erythrocytes and at sites of cell–cell contact in epithelial tissues.

While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension.

Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit comprises two distinct domains:

• The amino-terminal region is protease-resistant and globular in shape.
• The carboxy-terminal region is protease-sensitive, contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin.

Various adducin beta mRNAs, alternatively spliced at the 3′ end and/or internally spliced to encode different isoforms, have been described. The functions of all the different isoforms are not yet known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.