Thermo Fisher Scientific alpha Tubulin Polyclonal Antibody
Rabbit polyclonal antibody against human alpha Tubulin for WB, IHC, and ICC/IF applications. High specificity and broad species reactivity. Suitable for structural and cytoskeletal research. Supplied as liquid, affinity-purified, and unconjugated form.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Tubulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.63 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792305 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (97%), Xenopus laevis (98%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).
- Positive Control: 293T, A431, HeLa, HepG2.
- Centrifuge briefly prior to opening the vial.
Target Information
Alpha Tubulin is part of a heterodimer consisting of alpha and beta tubulin subunits. Each subunit has a molecular weight of approximately 55 kDa. The alpha-beta tubulin heterodimer forms the basic building block of microtubules, which serve as structural supports and transport lines within eukaryotic cells, and play a key role in mitosis.
Microtubules, composed of alpha and beta tubulins, perform essential and diverse cytoskeletal functions. Gamma tubulin is critical for microtubule nucleation. Multiple alpha and beta tubulin genes are highly conserved among species. The alpha tubulin gene is similar to the mouse and rat Tuba1 gene, with expression predominantly in differentiated neurologic cells.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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