Thermo Fisher Scientific eIF3g Polyclonal Antibody
eIF3g 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, Flow cytometry에 적합합니다. 인간 단백질에 반응하며, 서열 상 유사성으로 소, 마우스, 제브라피시 등에도 예측 반응합니다. 단백질 번역 개시 복합체 연구에 유용합니다.
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- PA525261
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | - |
| Flow Cytometry (Flow) | 1:10–1:50 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 209–238 amino acids from the central region of human EIF3G |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2542761 |
Product Specific Information
This antibody is predicted to react with bovine, mouse, rat, Xenopus, and zebrafish based on sequence homology.
Target Information
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis.
The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S preinitiation complex (43S PIC).
It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition.
The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
This subunit can bind 18S rRNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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