Thermo Fisher Scientific NSE Polyclonal Antibody
Rabbit polyclonal antibody targeting human Neuron Specific Enolase (NSE). Validated for WB and IHC applications. Ready-to-use format for paraffin sections. Suitable for detecting neuronal and neuroendocrine cells. For research use only.
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Thermo Fisher Scientific NSE Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Ready-to-use | 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the Human C-terminal region |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1959045 |
Product Specific Information
Recommended positive controls: IMR cells, Pancreas.
Target Information
Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. NSE is highly stable in biological fluids and diffuses into cerebrospinal fluid when neuronal membranes are injured.
NSE is one of three mammalian enolases (alpha, beta, gamma). The gamma-subunit is expressed primarily in neurons and neuroendocrine cells. Co-expression of NSE and chromogranin A is typical in neuroendocrine neoplasms.
Antibodies to NSE are useful for identifying neuronal cell bodies, developing neuronal lineage, and neuroendocrine cells. NSE release into CSF and blood serves as a biomarker of neuronal injury and is clinically used as a marker in neurological disorders such as stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Elevated NSE levels are also found in plasma and certain neoplasias, including pediatric neuroblastoma and small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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