Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (2G5)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:10-1:25

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Guinea pig, Hamster, Human, Mouse, Sheep, Pig, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2G5

Immunogen

Purified H-2Kb and H-2Db MHC-I molecules.

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• FITC
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_934633

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Mouse MHC Class I antibody, clone 2G5 recognizes a monomorphic epitope present on murine MHC class I molecules, expressed at varying levels on the majority of nucleated cells.

Target Information

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.