Thermo Fisher Scientific TIE2 Polyclonal Antibody, Biotin
TIE2(TEK) 단백질을 인식하는 Biotin 결합 토끼 폴리클로날 항체로, WB, IHC, ELISA, IP, Dot blot에 사용 가능. 친화 크로마토그래피로 정제되었으며, 0.5–1.5 mg/mL 농도의 액상 형태. 내피세포 특이적 수용체 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Dot Blot (DB) | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 540–590 on human Tyrosine-protein kinase receptor Tie-1 isoform 2 protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The TEK receptor tyrosine kinase (also known as TIE2) is expressed almost exclusively in endothelial cells. TEK signaling is critical for endothelial cell–smooth muscle cell communication during venous morphogenesis, and defects in TEK are linked to inherited venous malformations. The ligand angiopoietin-1 binds to this receptor, mediating a signaling pathway essential for embryonic vascular development. TIE2 expression increases in response to thyroid-stimulating hormone and agents that elevate intracellular cAMP. Hematopoietic stem cells expressing TIE2 are quiescent, antiapoptotic, and form a side population that adheres to osteoblasts within the bone marrow niche.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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