Thermo Fisher Scientific RNA Polymerase II Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunoprecipitation (IP)

2-10 µg/mg lysate

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

C-terminal repeat of Human RNA Polymerase II. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RNA pol II CTD,uniProtId:P24928-1,ncbiNodeId:9606,antigenRange:1970,antigenLength:1970,antigenImageFileName:A303-835A_RNA_pol_II_CTD_P24928-1_Goat.svg,antigenImageFileNamePDP:A303-835A_RNA_pol_II_CTD_P24928-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

phosphate/tris citrate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.