Thermo Fisher Scientific NEDD8 Polyclonal Antibody
다양한 면역학적 응용(IHC, ICC, ELISA)에 적합한 NEDD8 폴리클로날 항체. 사람, 마우스, 랫트 반응성. KLH 결합 합성 펩타이드(aa 31–81)로부터 유래. Protein A로 정제된 1 mg/mL 액상 항체로, -20°C에서 안정적 보관 가능.
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Thermo Fisher Scientific NEDD8 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human NEDD8 (amino acids 31–81) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
NEDD8 is a ubiquitin-like protein that plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires activation by the E1 complex (UBE1C-APPBP1) and linkage to the E2 enzyme (UBE2M). Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, promoting polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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