Takara Agrobacterium tumefaciens electrocompetent cells

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Applications

• Preparation of transgenic recombinant plants
• T-DNA transfer to plant cells
• Binary vector delivery
• Highly efficient plasmid transformation

Quality

Transformation efficiency was tested using 1 ng of pRI900 DNA. Colonies were selected using kanamycin and streptomycin plates. Transformation efficiency of >5 x 106 colonies/µg of pRI900 DNA was achieved. See Section IV. Protocol in the User Manual for more information.

Product citations

Hoekema, A. Hirsch, P.R. Hooykaas, P.J.J. Schilperoort, R.A. A binary plant vector strategy based on separation of vir_- and T-region of the _Agrobacterium tumefaciens Ti-plasmid._ Nature_ 303, 179–180.

Ooms, G. et al. _Octopine Ti-plasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region. _Plasmid 7:15–29 (1982).

Wen-Jun, S. & Forde, B.G. Efficient transformation of Agrobacterium spp. by high voltage electroporation. Nucleic Acids Res. 17(20):8385 (1989).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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