Takara Agrobacterium tumefaciens electrocompetent cells

Takara Agrobacterium tumefaciens Electrocompetent Cells

Applications

• Preparation of transgenic recombinant plants
• T-DNA transfer to plant cells
• Binary vector delivery
• Highly efficient plasmid transformation

Quality

Transformation efficiency was tested using 1 ng of pRI900 DNA. Colonies were selected using kanamycin and streptomycin plates.
Transformation efficiency of >5 × 10⁶ colonies/μg of pRI900 DNA was achieved.
See Section IV. Protocol in the User Manual for more information.

Product Citations

• Hoekema, A., Hirsch, P.R., Hooykaas, P.J.J., Schilperoort, R.A.
  A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid.
  Nature 303, 179–180.
• Ooms, G. et al.
  Octopine Ti-plasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region.
  Plasmid 7:15–29 (1982).
• Wen-Jun, S. & Forde, B.G.
  Efficient transformation of Agrobacterium spp. by high voltage electroporation.
  Nucleic Acids Res. 17(20):8385 (1989).

Additional Product Information

Please refer to the product's Certificate of Analysis for details on:

• Storage conditions
• Product components
• Technical specifications

The Kit Components List and Certificate of Analysis are available under the Documents tab.

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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