Thermo Fisher Scientific XPF Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human XPF. The exact sequence is proprietary. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:XPF,uniProtId:Q92889-1,ncbiNodeId:9606,antigenRange:916,antigenLength:916,antigenImageFileName:PA5-77973_XPF_Q92889-1_Rabbit.svg,antigenImageFileNamePDP:PA5-77973_XPF_Q92889-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2736808

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2

Predicted Reactivity: Mouse (84%), Chicken (83%), Bovine (88%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The structure specific ERCC 1 / XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells, XPG cleaves 3of the DNA lesion while the ERCC1 / XPF complex makes the 5 incision. ERCC1 / XPF probably plays a role in processing partially processed recombination intermediates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.