Thermo Fisher Scientific p75 NGF Receptor (extracellular) Polyclonal Antibody, Atto 488
p75 NGF 수용체(Extracellular) 인식 토끼 폴리클로날 항체로, Atto 488 형광 표지. 인간, 마우스, 랫트 시료에 반응하며 IHC(F), ICC/IF, Flow Cytometry에 적합. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | 1:60 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:20 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CEEIPGRWITRSTPPE corresponding to amino acid residues 188–203 of human p75NTR, extracellular stalk domain |
| Conjugate | Atto™ 488 |
| Excitation / Emission Max | 501 / 523 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution: Add 50 µL of double distilled water (DDW), depending on sample size.
- The antibody ships as a lyophilized powder at room temperature.
- Upon arrival, store at -20°C.
- Reconstituted solution can be stored at 4°C for up to 1 week.
- For long-term storage, aliquot and keep at -20°C.
- Avoid multiple freeze/thaw cycles.
- Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
Neurotrophic factors regulate the survival, differentiation, and maintenance of neurons in the peripheral and central nervous systems, as well as other neural crest-derived cell types. Developing sympathetic neurons are dependent on nerve growth factor (NGF) during target competition in vivo.
Antibodies specific to NGFR p75 are valuable for detecting and characterizing NGFR p75, aiding studies of nervous system development, plasticity, and repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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